Recombinant Human SYF2, His-tagged

• Cat.No.: SYF2-4732H
• Product Overview: Recombinant human SYF2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes a nuclear protein that interacts with cyclin D-type binding-protein 1, which is thought to be a cell cycle regulator at the G1/S transition. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA
• Molecular Mass: 20.5 kDa (173aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGS FPTSNSLLHG THVPSTEEID RMVIDLEKQI EKRDKYSRRR PYNDDADIDY INERNAKFNK KAERFYGKYT AEIKQNLERG TAV DYEKVKL LEISAEDAER WERKKKRKNP DLGFSDYAAA QLRQYHRLTK QIKPDMETYE RLREKHGEEF
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SYF2?SYF2 pre-mRNA-splicing factor [?Homo sapiens?(human) ]
• Official Symbol: SYF2
• Synonyms: SYF2; SYF2 homolog, RNA splicing factor (S. cerevisiae); CBPIN, CCNDBP1 interactor; pre-mRNA-splicing factor SYF2; DKFZp564O2082; fSAP29; functional spliceosome associated protein 29; NTC31; p29
• Gene ID: 25949
• mRNA Refseq: NM_015484
• Protein Refseq: NP_056299
• MIM: 607090
• UniProt ID: O95926
• Chromosome Location: 1p36.11
• Pathway: Spliceosome; Spliceosome, 35S U5-snRNP
• Function: poly(A) RNA binding