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Recombinant Human TAF15 293 Cell Lysate

Cat.No. : TAF15-1274HCL
  • Specification
  • Gene Information
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Description : Antigen standard for TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa (TAF15), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : TAF15 TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa [ Homo sapiens ]
Official Symbol : TAF15
Synonyms : TAF15; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TAF2N, TATA box binding protein (TBP) associated factor, RNA polymerase II, N, 68kD (RNA binding protein 56); TATA-binding protein-associated factor 2N; hTAFII68; Npl3; RBP56; TAF(II)68; RBP56/CSMF fusion; TBP-associated factor 15; 68 kDa TATA-binding protein-associated factor; TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TAF2N; TAFII68;
Gene ID : 8148
mRNA Refseq : NM_003487
Protein Refseq : NP_003478
MIM : 601574
UniProt ID : Q92804
Chromosome Location : 17q11.1-q11.2
Pathway : Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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