Recombinant Human TAGLN Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TAGLN-3264H
• Product Overview: TAGLN MS Standard C13 and N15-labeled recombinant protein (NP_001001522) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 22.6 kDa
• AA Sequence: MANKGPSYGMSREVQSKIEKKYDEE LEERLVEWIIVQCGPDVGRPDRGRL GFQVWLKNGVILSKLVNSLYPDGSK PVKVPENPPSMVFKQMEQVAQFLKA AEDYGVIKTDMFQTVDLFEGKDMAA VQRTLMALGSLAVTKNDGHYRGDPN WFMKKAQEHKREFTESQLQEGKHVI GLQMGSNRGASQAGMTGYGRPRQII STRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TAGLN transgelin [ Homo sapiens (human) ]
• Official Symbol: TAGLN
• Synonyms: TAGLN; transgelin; DKFZp686P11128; SM22; SM22 alpha; SMCC; TAGLN1; transgelin variant 2; WS3 10; SM22-alpha; 22 kDa actin-binding protein; smooth muscle protein 22-alpha; WS3-10; DKFZp686B01212
• Gene ID: 6876
• mRNA Refseq: NM_001001522
• Protein Refseq: NP_001001522
• MIM: 600818
• UniProt ID: Q01995