Recombinant Human TEK, Fc-His tagged

• Cat.No.: TEK-153H
• Product Overview: Recombinant Human TEK precursor extracellular domain (Met 1-Lys 745) (NP_000450.2), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: Human Cells
• Tag: Fc&His
• Protein Length: 1-745 a.a.
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
• Molecular Mass: The recombinant human Tie2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 970 amino acids and predicts a molecular mass of 108.5 kDa. As a result of glycosylation, the apparent molecular mass of rhTie2/Fc monomer is approximately 125-135 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publications:
  TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1 (2016)

Gene Information

• Gene Name: TEK TEK tyrosine kinase, endothelial [ Homo sapiens ]
• Official Symbol: TEK
• Gene ID: 7010
• mRNA Refseq: NM_000459
• Protein Refseq: NP_000450
• MIM: 600221
• UniProt ID: Q02763

Citation

TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1

Journal: Science Advances    PubMed ID: 27757426    Data: 2016/4/1

Authors: Mohammad B. Hossain, Rehnuma Shifat, Candelaria Gomez-Manzano

Article Snippet:In vitro kinase assays were conducted with 200 ng of purified glutathione S -transferase (GST)–tagged TIE2 active protein (SignalChem, no. T04-11G) or the His-tagged TIE2 with deleted kinase domain (Creative BioMart, no. TEK-153H) in kinase buffer [25 mM MOPS (pH 7.2), 12.5 mM β-glycerol phosphate, 20 mM MgCl 2 , 12.5 mM MnCl2, 2 mM EDTA (pH 8.0), 5 mM EGTA (pH 7.0), and 0.25 mM DTT] with the addition of 20 μM adenosine triphosphate (ATP) for cold reactions, and 10 μM ATP mixed with 10 μCi of [γ- 32 P]ATP for radioactive reactions.. The substrates were added to the reaction mixture and incubated at 37°C for 30 min.The substrates were added to the reaction mixture and incubated at 37°C for 30 min.