Active Recombinant Human TET2 Protein, His&Flag tagged

• Cat.No.: TET2-01H
• Product Overview: Recombinant TET2 (1129-2002) protein that includes amino acids 1129-2002 of human TET2 proteins (accession number NP_001120680.1) was expressed in Sf9 cells and contains an N-terminal 6×His Tag and FLAG-Tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag&His
• Protein Length: 1129-2002 aa
• Description: TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET2 plays an important role in normal myelopoiesis. Somatic loss-of-function mutations in TET2 gene frequently occur in patients with myeloid malignancies. TET2 loss increased hematopoietic stem cell self-renewal and myeloid transformation. TET2 promotes histone O-GlcNAcylation during gene transcription in embryonic stem cells. In addition, TET2 can be recruited to chromatin by CXXC domain protein IDAX. IDAX downregulates TET2 protein by activating caspase pathway.
• Form: Supplied in 25mM HEPES pH 7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.
• Molecular Mass: 102.7 kDa
• Purity: > 70% by SDS-PAGE
• Applications: Recombinant TET2 (1129-2002) protein is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling. TET Activity Assay Conditions: 250 nM of 30 bp DNA oligos (sequence: 5'-CAGTAACTGTGGTC(mC)GGTAACTGACTTGCA-3', contains a 5mCpG site) was incubated with 100 nM recombinant TET2 (1129-2002) protein in buffer containing 50 mM HEPES, pH 8.0, 100 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, 1 mM alpha-ketoglutarate, 1 mM ATP, 1 mM TCEP at 37 centigrade for 1 hr. Activity was detected by Dot-blot. This product has been used in the following publication(s): Shen, et. al., Nature. 2018 Feb 1;554(7690):123-127.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.2 μg/μL

Gene Information

• Gene Name: TET2 tet methylcytosine dioxygenase 2 [ Homo sapiens (human) ]
• Official Symbol: TET2
• Synonyms: TET2; tet methylcytosine dioxygenase 2; KIAA1546, tet oncogene family member 2; methylcytosine dioxygenase TET2; FLJ20032; tet oncogene family member 2; probable methylcytosine dioxygenase TET2; MDS; KIAA1546; MGC125715
• Gene ID: 54790
• mRNA Refseq: NM_001127208
• Protein Refseq: NP_001120680
• MIM: 612839
• UniProt ID: Q6N021