Recombinant Human TGFB1 Protein(Leu30-Arg278(C33S)), His-Avi-tagged, Biotinylated

• Cat.No.: TGFB1-3755H
• Product Overview: Biotinylated Recombinant Human TGFB1 Protein(Leu30-Arg278(C33S))(P01137) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu30-Arg278(C33S)
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137