Recombinant Human TGIF1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TGIF1-4324H
• Product Overview: TGIF1 MS Standard C13 and N15-labeled recombinant protein (NP_775300) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described.
• Molecular Mass: 29.7 kDa
• AA Sequence: MKGKKGIVAASGSETEDEDSMDIPLDLSSSAGSGKRRRRGNLPKESVQILRDWLYEHRYNAYPSEQEKALLSQQTHLSTLQVCNWFINARRRLLPDMLRKDGKDPNQFTISRRGAKISETSSVESVMGIKNFMPALEETPFHSCTAGPNPTLGRPLSPKPSSPGSVLARPSVICHTTVTALKDVPFSLCQSVGVGQNTDIQQIAAKNFTDTSLMYPEDTCKSGPSTNTQSGLFNTPPPTPPDLNQDFSGFQLLVDVALKRAAEMELQAKLTATRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TGIF1 TGFB-induced factor homeobox 1 [ Homo sapiens (human) ]
• Official Symbol: TGIF1
• Synonyms: TGIF1; TGFB-induced factor homeobox 1; HPE4, TGFB induced factor (TALE family homeobox), TGIF; homeobox protein TGIF1; homeobox protein TGIF; 5-TG-3 interacting factor; 5-TG-3-interacting factor 1; TALE homeobox TG-interacting factor; transforming growth factor-beta-induced factor; HPE4; TGIF; MGC5066; MGC39747
• Gene ID: 7050
• mRNA Refseq: NM_173208
• Protein Refseq: NP_775300
• MIM: 602630
• UniProt ID: Q15583