Recombinant Human THBS1 protein, His-tagged

• Cat.No.: THBS1-2657H
• Product Overview: Recombinant Human THBS1 aa. (Ser24~Thr221) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions
• Protein length: Ser24~Thr221
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: THBS1 thrombospondin 1 [ Homo sapiens (human) ]
• Official Symbol: THBS1
• Synonyms: THBS1; thrombospondin 1; TSP; THBS; TSP1; TSP-1; THBS-1; thrombospondin-1; thrombospondin-1p180; thrombospondin-p50
• Gene ID: 7057
• mRNA Refseq: NM_003246.3
• Protein Refseq: NP_003237.2
• UniProt ID: P07996