Recombinant Human THBS1 Protein, His-tagged

• Cat.No.: THBS1-355H
• Product Overview: Recombinant Human THBS1 fused with His tag at the C-terminus was produced in HEK293.

Specification

• Description: Thrombospondin-1 (TSP-1) is a 150-180kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1domains, 2 EGF-like domains, and 8 TSP type3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules. TSP-1 counteracts the angiogenic, hypotensive, and antithrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells(EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP).
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
• Protein length: Asn19-Pro1170
• AA sequence: NRIPESGGDNSVFDIFELTGAARKG SGRRLVKGPDPSSPAFRIEDANLIP PVPDDKFQDLVDAVR AEKGFLLLASLRQMKKTRGTLLALE RKDHSGQVFSVVSNGKAGTLDLSLT VQGKQHVVSVEEALL ATGQWKSITLFVQEDRAQLYIDCEK MENAELDVPIQSVFTRDLASIARLR IAKGGVNDNFQGVLQ NVRFVFGTTPEDILRNKGCSSSTSV LLTLDNNVVNGSSPAIRTNYIGHKT KDLQAICGISCDELS SMVLELRGLRTIVTTLQDSIRKVTE ENKELANELRRPPLCYHNGVQYRNN EEWTVDSCTECHCQN SVTICKKVSCPIMPCSNATVPDGEC CPRCWPSDSADDGWSPWSEWTSCST SCGNGIQQRGRSCDS LNNRCEGSSVQTRTCHIQECDKRFK QDGGWSHWSPWSSCSVTCGDGVITR IRLCNSPSPQMNGKP CEGEARETKACKKDACPINGGWGPW SPWDICSVTCGGGVQKRSRLCNNPT PQFGGKDCVGDVTEN QICNKQDCPIDGCLSNPCFAGVKCT SYPDGSWKCGACPPGYSGNGIQCTD VDECKEVPDACFNHN GEHRCENTDPGYNCLPCPPRFTGSQ PFGQGVEHATANKQVCKPRNPCTDG THDCNKNAKCNYLGH YSDPMYRCECKPGYAGNGIICGEDT DLDGWPNENLVCVANATYHCKKDNC PNLPNSGQEDYDKDG IGDACDDDDDNDKIPDDRDNCPFHY NPAQYDYDRDDVGDRCDNCPYNHNP DQADTDNNGEGDACA ADIDGDGILNERDNCQYVYNVDQRD TDMDGVGDQCDNCPLEHNPDQLDSD SDRIGDTCDNNQDID EDGHQNNLDNCPYVPNANQADHDKD GKGDACDHDDDNDGIPDDKDNCRLV PNPDQKDSDGDGRGD ACKDDFDHDSVPDIDDICPENVDIS ETDFRRFQMIPLDPKGTSQNDPNWV VRHQGKELVQTVNCD PGLAVGYDEFNAVDFSGTFFINTER DDDYAGFVFGYQSSSRFYVVMWKQV TQSYWDTNPTRAQGY SGLSVKVVNSTTGPGEHLRNALWHT GNTPGQVRTLWHDPRHIGWKDFTAY RWRLSHRPKTGFIRV VMYEGKKIMADSGPIYDKTYAGGRL GLFVFSQEMVFFSDLKYECRDPGGG GSHHHHHHHHHH
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100 μg/ml.
  Dissolve the lyophilized protein in ddH2O.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: THBS1 thrombospondin 1 [ Homo sapiens ]
• Official Symbol: THBS1
• Synonyms: THBS1; thrombospondin 1; thrombospondin-1; THBS; THBS 1; thrombospondin 1p180; TSP; TSP 1; TSP1; thrombospondin-1p180; TSP-1; THBS-1
• Gene ID: 7057
• mRNA Refseq: NM_003246
• Protein Refseq: NP_003237
• MIM: 188060
• UniProt ID: P07996