Recombinant Human THBS1 Protein, His-tagged

• Cat.No.: THBS1-355H
• Product Overview: Recombinant Human THBS1 fused with His tag at the C-terminus was produced in HEK293.

Specification

• Description: Thrombospondin-1 (TSP-1) is a 150-180kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1domains, 2 EGF-like domains, and 8 TSP type3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules. TSP-1 counteracts the angiogenic, hypotensive, and antithrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells(EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP).
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
• Protein length: Asn19-Pro1170
• AA sequence: NRIPESGGDNSVFDIFELTGAARKG SGRRLVKGPDPSSPAFRIEDANLIP PVPDDKFQDLVDAVR AEKGFLLLASLRQMKKTRGTLLALE RKDHSGQVFSVVSNGKAGTLDLSLT VQGKQHVVSVEEALL ATGQWKSITLFVQEDRAQLYIDCEK MENAELDVPIQSVFTRDLASIARLR IAKGGVNDNFQGVLQ NVRFVFGTTPEDILRNKGCSSSTSV LLTLDNNVVNGSSPAIRTNYIGHKT KDLQAICGISCDELS SMVLELRGLRTIVTTLQDSIRKVTE ENKELANELRRPPLCYHNGVQYRNN EEWTVDSCTECHCQN SVTICKKVSCPIMPCSNATVPDGEC CPRCWPSDSADDGWSPWSEWTSCST SCGNGIQQRGRSCDS LNNRCEGSSVQTRTCHIQECDKRFK QDGGWSHWSPWSSCSVTCGDGVITR IRLCNSPSPQMNGKP CEGEARETKACKKDACPINGGWGPW SPWDICSVTCGGGVQKRSRLCNNPT PQFGGKDCVGDVTEN QICNKQDCPIDGCLSNPCFAGVKCT SYPDGSWKCGACPPGYSGNGIQCTD VDECKEVPDACFNHN GEHRCENTDPGYNCLPCPPRFTGSQ PFGQGVEHATANKQVCKPRNPCTDG THDCNKNAKCNYLGH YSDPMYRCECKPGYAGNGIICGEDT DLDGWPNENLVCVANATYHCKKDNC PNLPNSGQEDYDKDG IGDACDDDDDNDKIPDDRDNCPFHY NPAQYDYDRDDVGDRCDNCPYNHNP DQADTDNNGEGDACA ADIDGDGILNERDNCQYVYNVDQRD TDMDGVGDQCDNCPLEHNPDQLDSD SDRIGDTCDNNQDID EDGHQNNLDNCPYVPNANQADHDKD GKGDACDHDDDNDGIPDDKDNCRLV PNPDQKDSDGDGRGD ACKDDFDHDSVPDIDDICPENVDIS ETDFRRFQMIPLDPKGTSQNDPNWV VRHQGKELVQTVNCD PGLAVGYDEFNAVDFSGTFFINTER DDDYAGFVFGYQSSSRFYVVMWKQV TQSYWDTNPTRAQGY SGLSVKVVNSTTGPGEHLRNALWHT GNTPGQVRTLWHDPRHIGWKDFTAY RWRLSHRPKTGFIRV VMYEGKKIMADSGPIYDKTYAGGRL GLFVFSQEMVFFSDLKYECRDPGGG GSHHHHHHHHHH
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100 μg/ml.
  Dissolve the lyophilized protein in ddH2O.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: THBS1 thrombospondin 1 [ Homo sapiens ]
• Official Symbol: THBS1
• Synonyms: THBS1; thrombospondin 1; thrombospondin-1; THBS; THBS 1; thrombospondin 1p180; TSP; TSP 1; TSP1; thrombospondin-1p180; TSP-1; THBS-1
• Gene ID: 7057
• mRNA Refseq: NM_003246
• Protein Refseq: NP_003237
• MIM: 188060
• UniProt ID: P07996

Reviews

05/14/2022

Quality exceeded my expectations.

09/08/2021

Satisfied customer, will return.

11/21/2020

Very pure, excellent results.

Q&As

In which tissues or cell types is THBS1 predominantly expressed?

THBS1 is widely expressed but is upregulated in response to injury or in certain disease states.

How is THBS1 implicated in cancer progression?

It can either promote or inhibit cancer progression, depending on the context.

How does THBS1 contribute to cell-matrix interactions?

It binds to extracellular matrix components, influencing cell attachment and signaling.

What role does THBS1 play in angiogenesis?

THBS1 acts as an angiogenesis inhibitor, hindering new blood vessel formation.

What therapeutic strategies are being explored targeting THBS1?

Therapeutic strategies include targeting THBS1 to modulate its effects in cancer and wound healing.

What is the primary function of THBS1 in cellular processes?

THBS1 modulates cell adhesion, proliferation, and migration.

Are there known genetic variations in THBS1 associated with specific diseases?

Genetic variations in THBS1 have been linked to cardiovascular diseases and cancer risk.