| Description : |
Thyroid-stimulating hormone (also known as TSH or thyrotropin) is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone, (TRH), which is manufactured in the hypothalamus and transported to the Anterior Pituitary gland, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release. The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH, When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop.TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit.The α (alpha) subunit is identical to that of human chorionic gonadotropin (HCG), luteinising hormone (LH), follicle-stimulating hormone (FSH). The β (beta) subunit is unique to TSH, and therefore determines its function. |
| Source : |
CHO cell |
| Species : |
Human |
| Enzyme Unit : |
9.5 IU per ampoule (by definition). Uncertainty: the assigned unitage is arbitrary and does not carry an uncertainty associated with it. When necessary, the uncertainty may be considered to be the coefficient of variation of the ampoule content, and was determined as ± 0.07% (cv) |
| Usage : |
No attempt should be made to weigh out any portion of the freeze-dried material prior to reconstitution. For practical purposes each ampoule contains the same quantity of thyroid- stimulating hormone. The entire content of each ampoule should be completely dissolved in an accurately measured amount of diluent. No attempt should be made to weigh out portions of the freeze-dried powder. Suitable diluents are PBS, saline and most assay buffers. If the contents are to be diluted extensively, the addition of 0.05 – 0.1% protein (HSA or BSA) is recommended to minimise adsorption. The use of water to reconstitute ampoule contents is not recommended. The material has not been sterilized and the ampoules contain no bacteriostat. |
| Notes : |
This preparation is not for administration to humans. The material is not of human or bovine origin. As with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratorys safety procedures. Such safety procedures should include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials, to avoid cuts. |
| Stability : |
Reference materials are held at Creative Biomart within assured, temperature- controlled storage facilities. Stability studies at elevated temperatures have shown that the material is suitably stable for shipment at ambient temperature without any effect on the assigned value. Reference Materials should be stored on receipt as indicated on the label. Once reconstituted, diluted or aliquoted, users should determine the stability of the material according to their own method of preparation, storage and use. Users who have data supporting any deterioration in the characteristics of any reference preparation are encouraged to contact Creative Biomart. |
| Storage : |
Unopened ampoules should be stored at -20°C. Please note: because of the inherent stability of lyophilized material, Creative Biomart may ship these materials at ambient temperature. |
