Recombinant Human TIE1 protein, His-tagged

• Cat.No.: TIE1-3965H
• Product Overview: Recombinant Human TIE1 protein(849-1138 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 849-1138 aa
• AA Sequence: NFGQVIRAMIKKDGLKMNAAIKMLK EYASENDHRDFAGELEVLCKLGHHP NIINLLGACKNRGYLYIAIEYAPYG NLLDFLRKSRVLETDPAFAREHGTA STLSSRQLLRFASDAANGMQYLSEK QFIHRDLAARNVLVGENLASKIADF GLSRGEEVYVKKTMGRLPVRWMAIE SLNYSVYTTKSDVWSFGVLLWEIVS LGGTPYCGMTCAELYEKLPQGYRME QPRNCDDEVYELMRQCWRDRPYERP PFAQIALQLGRMLEARKAYVNMSLF ENFTYAGIDATAEEA
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: TIE1 tyrosine kinase with immunoglobulin-like and EGF-like domains 1 [ Homo sapiens ]
• Official Symbol: TIE1
• Synonyms: TIE1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; TIE, tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; tyrosine-protein kinase receptor Tie-1; JTK14; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; TIE
• Gene ID: 7075
• mRNA Refseq: NM_001253357
• Protein Refseq: NP_001240286
• MIM: 600222
• UniProt ID: P35590