| Cat.No. : |
TIGIT-356H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human TIGIT Protein (Met22-Pro141, A103) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line. |
| Description : |
This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. |
| Source : |
CHO |
| Species : |
Human |
| Tag : |
C-Fc |
| Form : |
Disulfide-linked homodimer |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human TIGIT (A103) Fc Chimera that produces 50% optimal binding response is 40-200 ng/mL. |
| Molecular Mass : |
Predicted Molecular Mass: 40 kDa
SDS-PAGE: 40-53 kDa, reducing conditions |
| Protein Length : |
Met22-Pro141, A103 |
| N-terminal Sequence Analysis : |
Met22 |
| Endotoxin : |
< 0.10 EU/μg of the protein by the LAL method. |
| Purity : |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution : |
Reconstitute at 500 μg/mL in PBS. |
| Shipping : |
The product is shipped at ambient temperature. |
| Binding Activity : |
When Recombinant Human CD155/PVR is immobilized at 2.5 μg/mL, Recombinant Human TIGIT (A103) Fc Chimera binds with an ED50 of 40-200 ng/mL.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.