Active Recombinant Human TIMP2 protein, His-tagged, Low Endotoxin

• Cat.No.: TIMP2-448H
• Product Overview: Recombinant Human TIMP2 protein(P16035)(27-220 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 27-220 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Human TIMP-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TIMP2 TIMP metallopeptidase inhibitor 2 [ Homo sapiens ]
• Official Symbol: TIMP2
• Synonyms: TIMP2; TIMP metallopeptidase inhibitor 2; tissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; CSC 21K; TIMP-2; tissue inhibitor of metalloproteinases 2; CSC-21K
• Gene ID: 7077
• mRNA Refseq: NM_003255
• Protein Refseq: NP_003246
• MIM: 188825
• UniProt ID: P16035