Active Recombinant Human TLR4 Protein, His-tagged

• Cat.No.: TLR4-7275H
• Product Overview: Active Recombinant Human TLR4 Protein(Met1-Lys631) (O00206-1) was expressed in Insect Cells with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Form: Lyophilized from sterile 20mM PB, 500mM NaCl, pH 6.0, 10% glycerol.
• Bio-activity: 1.Immobilized Recombinant Human TLR4 / CD284 Protein (His Tag) at 1 μg/mL (100 μl/well) on His Tag Antibody, Mouse MAb precoated (5 μg/mL, 100 μL/well) can bind Anti-TLR4 Antibody, the EC50 is 4-15 ng/mL. 2.Immobilized Anti-TLR4 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human TLR4 / CD284 Protein (His Tag), the EC50 is 4-15 ng/mL.
• Molecular Mass: The secreted recombinant human TLR4 consists of 619 amino acids and predicts a molecular mass of 70.5 KDa. The apparent molecular mass of the protein is approximately 68 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 87 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TLR4 toll-like receptor 4 [ Homo sapiens ]
• Official Symbol: TLR4
• Synonyms: TLR4; toll-like receptor 4; CD284; hToll; TLR 4; homolog of Drosophila toll; TOLL; TLR-4; ARMD10
• Gene ID: 7099
• mRNA Refseq: NM_003266
• Protein Refseq: NP_003257
• UniProt ID: O00206