Recombinant Human TMPRSS2 Protein (106-492 aa), His-tagged

• Cat.No.: TMPRSS2-1856H
• Product Overview: Recombinant Human TMPRSS2 Protein (106-492 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions; spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 44.8 kDa
• Protein length: 106-492 aa
• AA Sequence: WKFMGSKCSNSGIECDSSGTCINPS NWCDGVSHCPGGEDENRCVRLYGPN FILQVYSSQRKSWHPVCQDDWNENY GRAACRDMGYKNNFYSSQGIVDDSG STSFMKLNTSAGNVDIYKKLYHSDA CSSKAVVSLRCIACGVNLNSSRQSR IVGGESALPGAWPWQVSLHVQNVHV CGGSIITPEWIVTAAHCVEKPLNNP WHWTAFAGILRQSFMFYGAGYQVEK VISHPNYDSKTKNNDIALMKLQKPL TFNDLVKPVCLPNPGMMLQPEQLCW ISGWGATEEKGKTSEVLNAAKVLLI ETQRCNSRYVYDNLITPAMICAGFL QGNVDSCQGDSGGPLVTSKNNIWWL IGDTSWGSGCAKAYRPGVYGNVMVF TDWIYRQMRADG
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Publication:
  An Enzymatic TMPRSS2 Assay for Assessment of Clinical Candidates and Discovery of Inhibitors as Potential Treatment of COVID-19 (2020)
  Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection (2021)
  Polyunsaturated ω-3 fatty acids inhibit ACE2-controlled SARS-CoV-2 binding and cellular entry (2021)
  Hesperidin Is a Potential Inhibitor against SARS-CoV-2 Infection (2021)
  Simultaneous Inhibition of SARS-CoV-2 Infectivity by a Specific Combination of Plant-derived Compounds (2021)
  FXa cleaves the SARS-CoV-2 spike protein and blocks cell entry to protect against infection with inferior effects in B.1.1.7 variant (2021)
  Suite of TMPRSS2 Assays for Screening Drug Repurposing Candidates as Potential Treatments of COVID-19 (2022)
  Electronic Supplementary Information for The ACE2 receptor accelerates but is not biochemically required for SARS-CoV-2 membrane fusion (2023)
  Phenolic compounds disrupt spike-mediated receptor-binding and entry of SARS-CoV-2 pseudo-virions (2021)
  Discovery and characterization of protease inhibitors that block SARS-CoV-2 infection (2023)
  Factor Xa cleaves SARS-CoV-2 spike protein to block viral entry and infection (2023)
  Peptidomimetic inhibitors of TMPRSS2 block SARS-CoV-2 infection in cell culture (2022)
  Molecular Interactions of Tannic Acid with Proteins Associated with SARS-CoV-2 Infectivity (2021)
  Improving the selectivity of 3-amidinophenylalanine-derived matriptase inhibitors (2022)

Gene Information

• Gene Name: TMPRSS2 transmembrane protease, serine 2 [ Homo sapiens ]
• Official Symbol: TMPRSS2
• Synonyms: TMPRSS2; PRSS10; epitheliasin; serine protease 10; PP9284; FLJ41954
• Gene ID: 7113
• mRNA Refseq: NM_001135099
• Protein Refseq: NP_001128571
• MIM: 602060
• UniProt ID: O15393