Recombinant Human TNF Protein, His-tagged TNF-001H

Recombinant Human TNF Protein, His-tagged

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Recombinant Human TNF Protein, His-tagged

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Cat.No. : TNF-001H
Product Overview : Recombinant Human TNF Protein (77-233 aa (partial, without signal peptide)) with 6xHis tag was expressed in E. coli.
Description : This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 18.4 kDa
Protein length : 77-233
Purity : >90% confirmed by 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-TNFa (WB).
Applications : ELISA, Western Blot, Antibody Production, Protein array
Quality Control Test : 15% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-TNFa (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry.
Storage : Prior to reconstitution, store the lyophilized protein at 2−8 centigrade. After reconstitution, store in working aliquots at −80 centigrade.
Expiry : All components are stable for 1 year of receipt, if stored properly.
Storage Buffer : 20mM Sodium Phosphate, 0.5M NaCl, pH 7.4. No preservatives added.
Shipping : Lyophilized at Ambient temperature
Figure :

Gene Name : TNF tumor necrosis factor [ Homo sapiens (human) ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
UniProt ID : P01375

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