Active Recombinant Human TNFRSF1B protein, hFc-tagged, Low Endotoxin

• Cat.No.: TNFRSF1B-238H
• Product Overview: Recombinant Human TNFRSF1B protein(P20333-1)(23-257 aa), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 23-257 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TNF alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 51.89 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFRSF1B tumor necrosis factor receptor superfamily, member 1B [ Homo sapiens ]
• Official Symbol: TNFRSF1B
• Synonyms: TNFRSF1B; tumor necrosis factor receptor superfamily, member 1B; TNFR2; tumor necrosis factor receptor superfamily member 1B; CD120b; p75; TNF R II; TNF R75; TNFBR; TNFR80; TNF-R2; TNF-RII; p75 TNF receptor; p80 TNF-alpha receptor; soluble TNFR1B variant 1; tumor necrosis factor receptor 2; tumor necrosis factor beta receptor; tumor necrosis factor receptor type II; tumor necrosis factor binding protein 2; TBPII; TNFR1B; TNF-R75; p75TNFR; TNF-R-II
• Gene ID: 7133
• mRNA Refseq: NM_001066
• Protein Refseq: NP_001057
• MIM: 191191
• UniProt ID: P20333