Active Recombinant Human TNFSF10 protein, mFc-tagged

• Cat.No.: TNFSF10-220H
• Product Overview: Recombinant Human TNFSF10 protein(Val 114 - Gly 281), fused with mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human Osteoprotegerin, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 0.8-13 ng/mL. Immobilized Human TRAIL R1, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 2-20 ng/mL. Immobilized Human TRAIL, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL R2, Fc Tag with a linear range of 0.6-20 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the N-terminus.The protein has a calculated MW of 46.4 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Val 114 - Gly 281
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ]
• Official Symbol: TNFSF10
• Synonyms: TNFSF10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10; Apo 2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; APO2L; Apo-2L
• Gene ID: 8743
• mRNA Refseq: NM_001190942
• Protein Refseq: NP_001177871
• MIM: 603598
• UniProt ID: P50591