Recombinant Human TNFSF10 protein, Fc-tagged
|Product Overview :||Recombinant Human TNFSF10 protein (Val114-Gly281) was fused to Mouse IgG2a Fc tag at N-terminus and expressed in human 293 cells (HEK293).|
|Description :||TRAIL, also known as TNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A and TNF superfamily member 10. The TRAIL gene as a drug target, induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). DcR1 functions as a TRAIL-neutralizing decoy-receptor. The cytoplasmic domain of DcR2 is functional and activates NFkappaB. In cells expressing DcR2, TRAIL binding therefore activates NFkappaB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.|
|Tag :||Mouse IgG2a Fc|
|Predicted N Terminal :||Glu|
|Form :||Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.|
|Molecular Mass :||The protein has a calculated MW of 46.4 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||168|
|Endotoxin :||Less than 0.1 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
|SDS-PAGE of TNFSF10-220H :||
Human TRAIL, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of TNFSF10-220H :||
Immobilized Human Osteoprotegerin, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 0.8-13 ng/mL (QC tested).
Immobilized Human TRAILR1, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 2-20 ng/mL (Routinely tested).
Immobilized Human TRAIL, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL R4, Fc Tag with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Immobilized Human TRAIL, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human TRAILR2, Fc Tag with a linear range of 0.6-20 ng/mL (Routinely tested).
|Gene Name :||TNFSF10|
|Official Symbol :||TNFSF10|
|Synonyms :||TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A; Apo-2 ligand|
|Gene ID :||8743|
|mRNA Refseq :||NM_003810.4|
|Protein Refseq :||NP_003801.1|
|UniProt ID :||P50591|
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