Recombinant Human TNFSF10 protein, Fc-tagged

PRODUCTS

Home / Products / Recombinant Proteins / Recombinant Human TNFSF10 protein, Fc-tagged

Recombinant Human TNFSF10 protein, Fc-tagged

TNFSF10 Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : TNFSF10-220H
Product Overview : Recombinant Human TNFSF10 protein (Val114-Gly281) was fused to Mouse IgG2a Fc tag at N-terminus and expressed in human 293 cells (HEK293).
Description : TRAIL, also known as TNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A and TNF superfamily member 10. The TRAIL gene as a drug target, induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). DcR1 functions as a TRAIL-neutralizing decoy-receptor. The cytoplasmic domain of DcR2 is functional and activates NFkappaB. In cells expressing DcR2, TRAIL binding therefore activates NFkappaB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.
Source : HEK293
Species : Human
Tag : Mouse IgG2a Fc
Predicted N Terminal : Glu
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Molecular Mass : The protein has a calculated MW of 46.4 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 168
Endotoxin : Less than 0.1 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Stability : This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
SDS-PAGE of TNFSF10-220H :

Human TRAIL, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of TNFSF10-220H :

Immobilized Human Osteoprotegerin, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 0.8-13 ng/mL (QC tested).

Immobilized Human TRAILR1, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 2-20 ng/mL (Routinely tested).

Immobilized Human TRAIL, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL R4, Fc Tag with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Immobilized Human TRAIL, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human TRAILR2, Fc Tag with a linear range of 0.6-20 ng/mL (Routinely tested).

Gene Name : TNFSF10
Official Symbol : TNFSF10
Synonyms : TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A; Apo-2 ligand
Gene ID : 8743
mRNA Refseq : NM_003810.4
Protein Refseq : NP_003801.1
MIM : 603598
UniProt ID : P50591

Online Inquiry


  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved.