Active Recombinant Human TNFSF11 protein, Avi-Fc-tagged, Biotinylated

• Cat.No.: TNFSF11-209H
• Product Overview: Biotinylated Recombinant Human TNFSF11 protein(Gly 64 - Asp 245), fused with Avi-Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Avi-Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Avitag,Fc Tag with a linear range of 0.4-13 ng/mL. Immobilized Human OPG, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Avitag,Fc Tag with a linear range of 0.3-10 ng/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag) at the N-terminus, followed by a human IgG1 Fc tag.The protein has a calculated MW of 49.2 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gly 64 - Asp 245
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• UniProt ID: O14788