|Product Overview:||Recombinant Human TNFSF11 Protein, Fc-tagged is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1). Predicted N-terminus: Pro. This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 46.9 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Recombinant Human TNFSF11 Protein, Fc-tagged with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized Human OPG, His Tag at 10 μg/mL (100 μL/well) can bind Recombinant Human TNFSF11 Protein, Fc-tagged with a linear range of 0.3-2 ng/mL (Routinely tested).
|Molecular Mass:||46.9 kDa|
|Protein length:||Gly 64 - Asp 245|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>90% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]|
|Synonyms:||TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; CD254; ODF; OPGL; RANKL; TRANCE; OPTB2; hRANKL2;|