Active Recombinant Human TNFSF18 protein, His-Flag-tagged, Low Endotoxin

• Cat.No.: TNFSF18-6854H
• Product Overview: Recombinant Human TNFSF18 protein(Q9UNG2)(Gln50-Ser177), fused with N-terminal His and Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 50-177 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GITR Ligand Trimer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF18 tumor necrosis factor (ligand) superfamily, member 18 [ Homo sapiens ]
• Official Symbol: TNFSF18
• Synonyms: TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18; AITRL; hGITRL; TL6; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; GITRL; MGC138237
• Gene ID: 8995
• mRNA Refseq: NM_005092
• Protein Refseq: NP_005083
• MIM: 603898
• UniProt ID: Q9UNG2