Recombinant Human TNFSF18 protein, monomeric hFc-tagged

• Cat.No.: TNFSF18-1508H
• Product Overview: Recombinant Human TNFSF18 protein(Q9UNG2)(Gln50-Ser177), fused with N-terminal monomeric hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-monomeric hFc
• Protein length: Gln50-Ser177
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GITR Ligand Trimer, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF18 tumor necrosis factor (ligand) superfamily, member 18 [ Homo sapiens ]
• Official Symbol: TNFSF18
• Synonyms: TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18; AITRL; hGITRL; TL6; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; GITRL; MGC138237
• Gene ID: 8995
• mRNA Refseq: NM_005092
• Protein Refseq: NP_005083
• MIM: 603898
• UniProt ID: Q9UNG2