Recombinant Human TNFSF4 Protein, hFc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: TNFSF4-490HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human TNFSF4 protein (Gln51-Leu183) was fused to human IgG1 Fc tag at N-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 42.4 kDa. The protein migrates as 53-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln51-Leu183
• N-terminal Sequence Analysis: Gly
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF4
• Official Symbol: TNFSF4
• Synonyms: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• Gene ID: 7292
• mRNA Refseq: NM_003326
• Protein Refseq: NP_003317
• MIM: 603594
• UniProt ID: P23510

Reviews

12/28/2022

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05/13/2018

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02/12/2018

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Q&As

How do genetic variations in TNFSF4 affect immune function?

Genetic changes in TNFSF4 affect immune regulation and autoimmune disease susceptibility.

What is the primary role of TNFSF4 in immune system regulation?

TNFSF4 (OX40L) regulates immune responses and T-cell activation.

How does TNFSF4 contribute to inflammatory responses?

It activates T-cells, stimulating inflammatory cytokine production.

What role does TNFSF4 play in autoimmune disorders?

TNFSF4 is implicated in autoimmune disease development by promoting immune cell activation.

How does TNFSF4 interact with other cytokines and receptors in the immune system?

TNFSF4 interacts with OX40 and other receptors, influencing immune signaling.

What potential therapeutic applications arise from targeting TNFSF4 in immune-related diseases?

Targeting TNFSF4 could treat autoimmune and inflammatory diseases by modulating the immune system.

What is the impact of altered TNFSF4 activity on chronic inflammation?

Altered TNFSF4 activity contributes to chronic inflammation.