Active Recombinant Human TNFSF9 protein, Avi&Fc-tagged, Biotinylated

• Cat.No.: TNFSF9-720H
• Product Overview: Biotinylated Recombinant Human TNFSF9 protein(NP_003802.1)(Arg71-Glu254), fused with Avi tag and Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 71-254 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Immobilized 4-1BB-Fch at 10 μg/mL (100 μL/well) can bind S3-Avi-Fc-TNFSF9, the EC50 of S3-Avi-Fc-TNFSF9 is 7.0-20 ng/mL.2. Measured by it's ability to induce IL-8 secretion by HT1080 transfected with human CD137. The ED50 for this effect is typically 0.25-1.5 μg/mL.
• Molecular Mass: The recombinant human TNFSF9 comprises 437 amino acids and has a calculated molecular mass of 47.98 kDa.
• Proteinlength: Arg71-Glu254
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: TNFSF9 tumor necrosis factor (ligand) superfamily, member 9 [ Homo sapiens ]
• Official Symbol: TNFSF9
• Synonyms: TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9; 4 1BB L; homolog of mouse 4 1BB L; receptor 4 1BB ligand; 4-1BBL; 4-1BB ligand; receptor 4-1BB ligand; homolog of mouse 4-1BB-L; CD137L; 4-1BB-L
• Gene ID: 8744
• mRNA Refseq: NM_003811
• Protein Refseq: NP_003802
• MIM: 606182
• UniProt ID: P41273