Active Recombinant Human TNNI3 Protein, His-tagged
Cat.No. : | TNNI3-873H |
Product Overview : | Active Recombinant Human TNNI3 Protein(2-210 aa), fused with N-terminal His tag, was expressed in HEK293. |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
Protein Length : | 2-210 aa |
Form : | PBS, pH7.4, containing 5% Trehalose. |
Bio-activity : | Cardiac Troponin I (cTnI), as a cytoplasmic protein, is one of the most important regulatory proteins part of the troponin-tropomyosin complex localized on the actin (thin) myofilaments in cardiac myocytes.It is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.Besides,Actin Alpha 1, Cardiac Muscle (ACTC1) has been identified as an interactor of cTnI, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human cTnI and recombinant human ACTC1. Briefly, biotin-linked cTnI were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μ l were then transferred to ACTC1-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of cTnI and ACTC1 was shown, the EC50 for this effect is 0.066ug/mL. |
Molecular Mass : | 25.5kDa |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | >90% |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month. |
Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | TNNI3 troponin I type 3 (cardiac) [ Homo sapiens ] |
Official Symbol | TNNI3 |
Synonyms | TNNI3; troponin I type 3 (cardiac); troponin I, cardiac; troponin I, cardiac muscle; CMH7; TNNC1; RCM1; cTnI; CMD2A; CMD1FF; MGC116817; |
Gene ID | 7137 |
mRNA Refseq | NM_000363 |
Protein Refseq | NP_000354 |
MIM | 191044 |
UniProt ID | P19429 |
◆ Recombinant Proteins | ||
Tnni3-17M | Recombinant Mouse Tnni3 protein, His-tagged | +Inquiry |
TNNI3-2684H | Recombinant Human TNNI3 protein(11-130 aa) | +Inquiry |
Tnni3-2676R | Recombinant Rat Tnni3 protein, His-tagged | +Inquiry |
TNNI3-008H | Recombinant Human TNNI3 Protein | +Inquiry |
TNNI3-02H | Recombinant Human TNNI3 Protein, His-Tagged | +Inquiry |
◆ Native Proteins | ||
TNNI3-223H | Native Human Troponin I Type 3 (Cardiac) | +Inquiry |
Tnni3-7423M | Native Mouse Tnni3 Protein | +Inquiry |
TNNI3-01H | Native Human TNNI3 Protein | +Inquiry |
TNNI3-221H | Native Human TNNI3 | +Inquiry |
◆ Cell & Tissue Lysates | ||
TNNI3-1803HCL | Recombinant Human TNNI3 cell lysate | +Inquiry |
Not For Human Consumption!
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