Active Recombinant Human TNNI3 Protein, His-tagged

• Cat.No.: TNNI3-873H
• Product Overview: Active Recombinant Human TNNI3 Protein(2-210 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 2-210 aa
• Form: PBS, pH7.4, containing 5% Trehalose.
• Bio-activity: Cardiac Troponin I (cTnI), as a cytoplasmic protein, is one of the most important regulatory proteins part of the troponin-tropomyosin complex localized on the actin (thin) myofilaments in cardiac myocytes.It is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.Besides,Actin Alpha 1, Cardiac Muscle (ACTC1) has been identified as an interactor of cTnI, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human cTnI and recombinant human ACTC1. Briefly, biotin-linked cTnI were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μ l were then transferred to ACTC1-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of cTnI and ACTC1 was shown, the EC50 for this effect is 0.066ug/mL.
• Molecular Mass: 25.5kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TNNI3 troponin I type 3 (cardiac) [ Homo sapiens ]
• Official Symbol: TNNI3
• Synonyms: TNNI3; troponin I type 3 (cardiac); troponin I, cardiac; troponin I, cardiac muscle; CMH7; TNNC1; RCM1; cTnI; CMD2A; CMD1FF; MGC116817
• Gene ID: 7137
• mRNA Refseq: NM_000363
• Protein Refseq: NP_000354
• MIM: 191044
• UniProt ID: P19429