Recombinant Human TNNT1 protein, His-tagged

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Recombinant Human TNNT1 protein, His-tagged

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Cat.No. : TNNT1-7947H
Product Overview : Recombinant Human TNNT1 aa. (Met1~Gly259) fused with N-terminal His tag was produced in E. coli cells.
Description : This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 40kDa as determined by SDS-PAGE reducing conditions.
Protein length : Met1~Gly259
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >92%
Characteristic : The isoelectric point is 5.7.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage Buffer : PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Reconstitution : Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : TNNT1 troponin T1, slow skeletal type [ Homo sapiens (human) ]
Official Symbol : TNNT1
Synonyms : TNNT1; troponin T1, slow skeletal type; ANM; TNT; NEM5; STNT; TNTS; troponin T, slow skeletal muscle; slow skeletal muscle troponin T; troponin T type 1 (skeletal, slow); troponin-T1, skeletal, slow
Gene ID : 7138
mRNA Refseq : NM_001126132.2
Protein Refseq : NP_001119604.1
UniProt ID : P13805

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