Recombinant Human TNNT2 protein, His & GST-tagged

• Cat.No.: TNNT2-7950H
• Product Overview: Recombinant Human TNNT2 aa. (Ser2~Ile100 (Accession # P45379)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.5kDa
• Protein length: Ser2~Ile100 (Accession # P45379)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: TNNT2 troponin T2, cardiac type [ Homo sapiens (human) ]
• Official Symbol: TNNT2
• Synonyms: TNNT2; troponin T2, cardiac type; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6; troponin T, cardiac muscle; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; troponin T type 2 (cardiac); truncated cardiac troponin T
• Gene ID: 7139
• mRNA Refseq: NM_000364.3
• Protein Refseq: NP_000355.2
• UniProt ID: P45379