Active Recombinant Human TPBG Protein, Fc-tagged

• Cat.No.: TPBG-32H
• Product Overview: Recombinant human TPBG-Fc fusion is expressed as a 561 amino acid protein consisting of Ser32 - Ser355 region of TPBG and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing condition.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human TPBG, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-TPBG Antibody, Human IgG1 with a linear range of 0.5-8 ng/mL.
• Molecular Mass: The protein has a calculated MW of 61.8 kDa. The protein migrates as 80-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Ser 32 - Ser 355
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TPBG trophoblast glycoprotein [ Homo sapiens ]
• Official Symbol: TPBG
• Synonyms: TPBG; trophoblast glycoprotein; 5T4; 5T4 AG; 5T4 oncofetal antigen; 5T4 oncotrophoblast glycoprotein; 5T4 oncofetal trophoblast glycoprotein; M6P1; 5T4AG
• Gene ID: 7162
• mRNA Refseq: NM_001166392
• Protein Refseq: NP_001159864
• MIM: 190920
• UniProt ID: Q13641