Recombinant Human TPO protein(257 Ser/Ala, 725 Pro/Thr), His-tagged

• Cat.No.: TPO-116H
• Product Overview: Recombinant Human TPO protein(P07202-1)(Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-846 aa,257 Ser/Ala, 725 Pro/Thr
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
• Molecular Mass: The secreted recombinant human TPO comprises 842 amino acids and has a predicted molecular mass of 93.8 kDa. ? The apparent molecular mass of rh TPO (1-846) is approximately 90-100 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TPO thyroid peroxidase [ Homo sapiens ]
• Official Symbol: TPO
• Synonyms: TPO; thyroid peroxidase; TPX; thyroperoxidase; thyroid microsomal antigen; MSA; TDH2A
• Gene ID: 7173
• mRNA Refseq: NM_000547
• Protein Refseq: NP_000538
• MIM: 606765
• UniProt ID: P07202