Recombinant Human TRMU Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TRMU-5913H
• Product Overview: TRMU MS Standard C13 and N15-labeled recombinant protein (NP_060476) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This nuclear gene encodes a mitochondrial tRNA-modifying enzyme. The encoded protein catalyzes the 2-thiolation of uridine on the wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln), resulting in the formation of 5-taurinomethyl-2-thiouridine moieties. Mutations in this gene may cause transient infantile liver failure. Polymorphisms in this gene may also influence the severity of deafness caused by mitochondrial 12S ribosomal RNA mutations. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 47.6 kDa
• AA Sequence: MQALRHVVCALSGGVDSAVAALLLR RRGYQVTGVFMKNWDSLDEHGVCTA DKDCEDAYRVCQILDIPFHQVSYVK EYWNDVFSDFLNEYEKGRTPNPDIV CNKHIKFSCFFHYAVDNLGADAIAT GHYARTSLEDEEVFEQKHVKKPEGL FRNRFEVRNAVKLLQAADSFKDQTF FLSQVSQDALRRTIFPLGGLTKEFV KKIAAENRLHHVLQKKESMGMCFIG KRNFEHFLLQYLQPRPGHFISIEDN KVLGTHKGWFLYTLGQRANIGGLRE PWYVVEKDSVKGDVFVAPRTDHPAL YRDLLRTSRVHWIAEEPPAALVRDK MMECHFRFRHQMALVPCVLTLNQDG TVWVTAVQAVRALATGQFAVFYKGD ECLGSGKILRLGPSAYTLQKGQRRA GMATESPSDSPEDGPGLSPLLTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TRMU tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase [ Homo sapiens (human) ]
• Official Symbol: TRMU
• Synonyms: TRMU; tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase; TRMT, tRNA (5 methylaminomethyl 2 thiouridylate) methyltransferase; mitochondrial tRNA-specific 2-thiouridylase 1; FLJ10140; MTO2; MTO2 homolog; mitochondrial 5-methylaminomethyl-2-thiouridylate-methyltransferase; MTU1; TRMT; TRMT1; TRNT1; MGC99627
• Gene ID: 55687
• mRNA Refseq: NM_018006
• Protein Refseq: NP_060476
• MIM: 610230
• UniProt ID: O75648