Recombinant Human TSC2, His-tagged

• Cat.No.: TSC2-31631TH
• Product Overview: Recombinant fragment, corresponding to amino acids 1396-1725 of Human Tuberin Isoform 5 with N terminal His tag, MWt 38kDa.

Specification

• Description: Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
• Form: Lyophilised:Reconstitute with 78 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: ISDSAPSRRGKRVERDALKSRATAS NAEKVPGINPSFVFL QLYHSPFFGDESNKPILLPNESQSF ERSVQLLDQIPSY DTHKIAVLYVGEGQSNSELAILSNE HGSYRYTEFLTGL GRLIELKDCQPDKVYLGGLDVCGED GQFTYCWHDDIMQ AVFHIATLMPTKDVDKHRCDKKRHL GNDFVSIVYNDSGEDFKLGTIKGQF NFVHVIVTPLDYECNLVSLQCRKDM EGL VDTSVAKIVSDRNLPFVARQMALHA NMASQVHHSRSNP TDIYPSKWIARLRHIKRLRQRICEE AAYSNPSLPLVHP PSHSKAPAQTPAEPTPGYEVGQ
• Sequence Similarities: Contains 1 Rap-GAP domain.

Gene Information

• Gene Name: TSC2 tuberous sclerosis 2 [ Homo sapiens ]
• Official Symbol: TSC2
• Synonyms: TSC2; tuberous sclerosis 2; TSC4; tuberin; LAM
• Gene ID: 7249
• mRNA Refseq: NM_000548
• Protein Refseq: NP_000539
• MIM: 191092
• Uniprot ID: P49815
• Chromosome Location: 16p13.3
• Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem; Downstream signaling of activated FGFR, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem
• Function: 14-3-3 protein binding; GTPase activator activity; protein binding; protein heterodimerization activity; protein homodimerization activity