Recombinant Human TSH therapeutic protein(Thyrotropin alfa)

• Cat.No.: TSH-P036H
• Product Overview: The therapeutic protein is a recombinant form of thyroid stimulating hormone used in performing certain tests in patients who have or have had thyroid cancer. It is also used along with a radioactive agent to destroy remaining thyroid tissue in certain patients who have had their thyroid gland removed because of thyroid cancer. It is a heterodimeric glycoprotein comprised of two non-covalently linked subunits, an alpha subunit of 92 amino acid residues containing two N-linked glycosylation sites and a beta subunit of 112 residues containing one N-linked glycosylation site. The alpha subunit is nearly identical to that of human chorionic gonadotropin (hCG), luteinizing hormone (LH), and follicle-stimulating hormone (FSH). The alpha subunit is thought to be the effector region responsible for stimulation of adenylate cyclase (involved the generation of cAMP). The beta subunit (TSHB) is unique to?TSH, and therefore determines its receptor specificity. The amino acid sequence of it is identical to that of human pituitary thyroid stimulating hormone.

Specification

• Description: The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. The expression product is the active ingredient of Thyrogen, Gonal-F, Metrodin HP and Puregon.
• Species: Human
• Molecular Mass: 22.7 kDa
• AA Sequence: >Alpha chainAPDVQDCPECTLQENPFFSQ PGAPILQCMGCCFSRAYPTPLRSKK TMLVQKNVTSESTCCVAKSYNRVTV MGGFKVENHTACHCSTCYYHKS>Beta chainFCIPTEYTMHIERRECAYCL TINTTICAGYCMTRDINGKLFLPKY ALSQDVCTYRDFIYRTVEIPGCPLH VAPYFSYPVALSCKCGKCNTDYSDC IHEAIKTNYCTKPQKSY
• Endotoxin: < 1.0 EU per μg of the protein
• Purity: >95%
• Storage: Can be stored at +4 centigrade short term (1-2weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoidrepeated freezing and thawing cycles.
• Tag: Non
• Alias: TSH; Recombinant Human Thyroid Stimulating Hormone; Recombinant thyrotropin alfa; rhTSH; rTSH; Thyrotropin alfa
• Brand Names: Thyrogen

Reviews

07/11/2022

Good for Bimo-lecular fluorescence complementation control.

06/01/2020

TSH manufacturer's products are consistently of the highest quality, with a strong commitment to purity, potency, and consistency.

05/17/2020

Work great in Western blot analysis.

12/02/2019

The purity is truly exceptional, with no visible impurities or contaminants.

03/18/2019

TSH is ideal for Western-blot analysis, with strong bands and clear specificity.

04/09/2018

Can save materials and shorten the experiment time in luci-ferase complementation assay.

04/03/2018

It was accurately quantified in expression analysis.

03/11/2017

It is appropriate for T cell killing analysis.

Q&As

What the TSHR signal transduction, regulation, and maturation?

The TSHR, long known to signal via cAMP, can induce both the phospholipase C (PLC) and the protein kinase A signal transduction systems. Intracellular Ca2+ and PLC regulate iodide efflux, H2O2 production, and thyroglobulin iodination, while adenylate cyclase and cAMP regulate iodide uptake and transcription of thyroglobulin (Tg), thyroid peroxidase (TPO), and the sodium-iodide symporter.

Why the puzzling size diversity of TSHRs detected by western blotting?

Presumably by protein disulfide isomerase leading to release of the α subunit from the membrane-bound receptor.

What is associated with a high TSH value?

High-normal TSH-values are associated with an adverse lipid profile, high blood pressure, high body mass index (BMI), metabolic syndrome and fatal coronary heart disease.

How is TSH level regulated in brain?

It is known that the majority of the metabolically active T3 in the brain is not derived from the circulation, but produced locally by the effect of D2 on T4. Locally produced T3 largely acts on the hypothalamus and pituitary glands to control TSH levels.

Are TSH receptors expressed in places other than the thyroid gland?

Thyroid stimulating hormone receptors have been identified in mammalian heart, bone, kidneys, brain, skin, adipose, hair follicles, thymus, lymphocytes, astrocyte cultures and in fish testes.

How can thyroid cancer be treated by inhibiting thyrotropin?

Levothyroxine is recommended for long-term TSH suppression.

What cells or organs does TSH work on?

Including thyroid,lymphocytes, the pituitary, thymus, testes, kidney, brain, adipose tissue, and fibroblasts.

What is the biological function of thyroid stimulating hormone (TSH)?

The only established biologic function of thyroid stimulating hormone (TSH) is to regulate the synthesis and secretion of thyroid hormone from thyroid follicular cells.

How to achieve TSH transcription?

In response to low circulating thyroid hormone levels, the basal medial hypothalamus releases thyrotropin-releasing hormone (TRH) which then stimulates production of thyroid-stimulating hormone (TSH) from the thyrotropes of the anterior pituitary gland.

Besides TSH, what other substances can stimulate the release of prolactin?

Other substances like serotonin, gonadotropin-releasing hormone, and estrogen can also stimulate prolactin release.

What is the role of TSH protein in thyroid cancer?

In thyroid cancer, the TSH protein may have a role in promoting the proliferation and growth of cancer cells. Therefore, for some high-risk groups, such as patients with postoperative thyroidectomy, inhibition of TSH protein production and activity can reduce the risk of cancer recurrence.

What factors regulate the secretion of TSH protein?

The secretion of TSH protein is regulated by a variety of factors, including negative feedback regulation of the hypothalamic-pituitary-thyroid axis, thyroxine levels, and the regulatory mechanism of anterior pituitary cells.

Does the TSH protein have polymorphisms?

The polymorphisms of the TSH protein are partly related to the genetic variation of individuals. TSH proteins of different genotypes may affect an individual's sensitivity to TSH and regulation of thyroid function.

How does TSH protein regulate thyroid function?

TSH protein activates intracellular signaling pathways by binding to TSH receptors on the surface of thyroid cells, promoting thyroxine synthesis and secretion. It enhances the production of thyroxine by positively regulating iodine uptake, thyroglobulin synthesis, and thyroid peroxidase activity.

What are the applications of TSH protein in clinical diagnosis?

The TSH protein plays a key role in clinical diagnosis. By measuring serum TSH levels, it is possible to assess whether the thyroid gland is functioning normally for diagnosis and follow-up of thyroid-related diseases.

Does the TSH protein interact with other proteins?

Yes, the TSH protein can interact with TSH receptors on the surface of thyroid cells, triggering a series of signaling. In addition, TSH protein interacts with other cytokines and regulators to regulate thyroid function.