Recombinant Human TSLP protein, His-Avi-tagged, Biotinylated

• Cat.No.: TSLP-3191H
• Product Overview: Biotinylated Recombinant Human TSLP protein(Q969D9-1)(Tyr29-Gln159(R127A,R130A)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Tyr29-Gln159(R127A,R130A)
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin

Gene Information

• Gene Name: TSLP thymic stromal lymphopoietin [ Homo sapiens ]
• Official Symbol: TSLP
• Synonyms: TSLP; thymic stromal lymphopoietin
• Gene ID: 85480
• mRNA Refseq: NM_033035
• Protein Refseq: NP_149024
• MIM: 607003
• UniProt ID: Q969D9