Recombinant Human TSLP protein, His-tagged
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|Product Overview :||Recombinant Human TSLP protein (Tyr29-Gln159), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.|
|Form :||Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 16.8 kDa. The protein migrates as 8 kDa and 14-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||131|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of TSLP-372H :||
Human TSLP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of TSLP-372H :||
Immobilized Human TSLPR, Fc Tag at 5 μg/mL (100 μL/well) can bind Human TSLP, His Tag with a linear range of 1.6-13 ng/mL (QC tested).
Immobilized Human TSLP, His Tag at 1 μg/mL (100 μL/well) can bind Anti-TSLP MAb, Human IgG2 with a linear range of 0.5-8 ng/mL (Routinely tested).
|Bioactivity-SPR of TSLP-372H :||
Captured Human TSLP, His Tag on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag with an affinity constant of 1.81 nM as determined in SPR assay (Routinely tested).
|Gene Name :||TSLP|
|Official Symbol :||TSLP|
|Synonyms :||TSLP; thymic stromal lymphopoietin|
|Gene ID :||85480|
|mRNA Refseq :||NM_033035|
|Protein Refseq :||NP_149024|
|UniProt ID :||Q969D9|
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