Recombinant Human TTL Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human TTL Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : TTL-5778H
Product Overview : TTL MS Standard C13 and N15-labeled recombinant protein (NP_714923) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin. Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 43.2 kDa
AA Sequence : MYTFVVRDEN SSVYAEVSRL LLATGHWKRL RRDNPRFNLM LGERNRLPFG RLGHEPGLVQ LVNYYRGADK LCRKASLVKL IKTSPELAES CTWFPESYVI YPTNLKTPVA PAQNGIQPPI SNSRTDEREF FLASYNRKKE DGEGNVWIAK SSAGAKGEGI LISSEASELL DFIDNQGQVH VIQKYLEHPL LLEPGHRKFD IRSWVLVDHQ YNIYLYREGV LRTASEPYHV DNFQDKTCHL TNHCIQKEYS KNYGKYEEGN EMFFKEFNQY LTSALNITLE SSILLQIKHI IRNCLLSVEP AISTKHLPYQ SFQLFGFDFM VDEELKVWLI EVNGAPACAQ KLYAELCQGI VDIAISSVFP PPDVEQPQTQ PAAFIKLTRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : TTL tubulin tyrosine ligase [ Homo sapiens (human) ]
Official Symbol : TTL
Synonyms : TTL; tubulin tyrosine ligase; tubulin--tyrosine ligase; MGC46235; 2410003M22Rik; tubulin-tyrosine ligase;
Gene ID : 150465
mRNA Refseq : NM_153712
Protein Refseq : NP_714923
MIM : 608291
UniProt ID : Q8NG68

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