Recombinant Human TXNRD2 Protein

• Cat.No.: TXNRD2-22H
• Product Overview: Recombinant Human Thioredoxin Reductase 2 (Non-tagged; No signal peptide; 488 residues)

Specification

• Species: Human
• Tag: Non
• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.
• Purity: ≥ 95% as judged by gel analysis
• Unit Definition: 1 U of enzyme reduces 1 μmol DTNB per min in 0.5 mL standard DTNB assay containing 2.5 mM DTNB and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5)
• Applications: DTNB assay; Insulin-coupled Thioredoxin assay; Inhibitor screening; etc.
• Notes: Centrifuge the tube briefly before opening the lid
• Storage: Store at -20 centigrade upon delivery.
• Concentration: 1 mg/mL
• Storage Buffer: TE buffer with 50% glycerol.
• Shipping: Shipped in cold pack by courier service.

Gene Information

• Gene Name: TXNRD2 thioredoxin reductase 2 [ Homo sapiens (human) ]
• Official Symbol: TXNRD2
• Synonyms: TXNRD2; thioredoxin reductase 2; TR; TR3; SELZ; GCCD5; TRXR2; TR-BETA; thioredoxin reductase 2, mitochondrial; selenoprotein Z; thioredoxin reductase 3; thioredoxin reductase TR3; thioredoxin reductase beta; EC 1.8.1.9
• Gene ID: 10587
• mRNA Refseq: NM_006440
• Protein Refseq: NP_006431
• MIM: 606448
• UniProt ID: Q9NNW7