| Description : |
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
111.7 kDa |
| AA Sequence : |
MDALDASKLLDEELYSRQLYVLGSP AMQRIQGARVLVSGLQGLGAEVAKN LVLMGVGSLTLHDPHPTCWSDLAAQ FLLSEQDLERSRAEASQELLAQLNR AVQVVVHTGDITEDLLLDFQVVVLT AAKLEEQLKVGTLCHKHGVCFLAAD TRGLVGQLFCDFGEDFTVQDPTEAE PLTAAIQHISQGSPGILTLRKGANT HYFRDGDLVTFSGIEGMVELNDCDP RSIHVREDGSLEIGDTTTFSRYLRG GAITEVKRPKTVRHKSLDTALLQPH VVAQSSQEVHHAHCLHQAFCALHKF QHLHGRPPQPWDPVDAETVVGLARD LEPLKRTEEEPLEEPLDEALVRTVA LSSAGVLSPMVAMLGAVAAQEVLKA ISRKFMPLDQWLYFDALDCLPEDGE LLPSPEDCALRGSRYDGQIAVFGAG FQEKLRRQHYLLVGAGAIGCELLKV FALVGLGAGNSGGLTVVDMDHIERS NLSRQFLFRSQDVGRPKAEVAAAAA RGLNPDLQVIPLTYPLDPTTEHIYG DNFFSRVDGVAAALDSFQARRYVAA RCTHYLKPLLEAGTSGTWGSATVFM PHVTEAYRAPASAAASEDAPYPVCT VRYFPSTAEHTLQWARHEFEELFRL SAETINHHQQAHTSLADMDEPQTLT LLKPVLGVLRVRPQNWQDCVAWALG HWKLCFHYGIKQLLRHFPPNKVLED GTPFWSGPKQCPQPLEFDTNQDTHL LYVLAAANLYAQMHGLPGSQDWTAL RELLKLLPQPDPQQMAPIFASNLEL ASASAEFGPEQQKELNKALEVWSVG PPLKPLMFEKDDDSNFHVDFVVAAA SLRCQNYGIPPVNRAQSKRIVGQII PAIATTTAAVAGLLGLELYKVVSGP RPRSAFRHSYLHLAENYLIRYMPFA PAIQTFHHLKWTSWDRLKVPAGQPE RTLESLLAHLQEQHGLRVRILLHGS ALLYAAGWSPEKQAQHLPLRVTELV QQLTGQAPAPGQRVLVLELSCEGDD EDTAFPPLHYELTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
