Recombinant Human UBAMC Protein
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|Product Overview :||Recombinant Human UBAMC Protein is produced by Bacteria expression system.|
|Description :||Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.|
|Molecular Mass :||9 kDa|
|Purity :||> 95 % by SDS-PAGE|
|Notes :||Avoid mixing with compound or buffer containing free amino group.|
|Storage :||Store at -80 centigrade; avoid multiple freeze-thaw cycles.|