Recombinant Human UBAMC Protein

• Cat.No.: UBAMC-383H
• Product Overview: Recombinant Human UBAMC Protein is produced by Bacteria expression system.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
• Form: DMSO
• Molecular Mass: 9 kDa
• Purity: > 95 % by SDS-PAGE
• Notes: Avoid mixing with compound or buffer containing free amino group.
• Storage: Store at -80 centigrade; avoid multiple freeze-thaw cycles.

Gene Information

• Synonyms: Ubiquitin-AMC