Recombinant Human UBE2Q2, His-tagged

• Cat.No.: UBE2Q2-101H
• Product Overview: Recombinant human UBE2Q2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: UBE2Q2 also known as Ubiquitin-conjugating enzyme E2 Q2 isoform1. UBE2Q2 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys-48-linked polyubiquitination. This protein detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium. It belongs to the ubiquitin-conjugating enzyme family.
• Form: Liquid. In Phosphate Buffer Saline (pH7.4) containing 30% glycerol, 1mM DTT
• Molecular Mass: 45.2kDa (398aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVSGLK AELKFLASIF DKNHERFRIV SWKLDELHCQ FLVPQQGSPH SLPPPLTLHC NITESYPSSS PIWFVDSEDP NLTSVLERLE DTKNNNLLRQ QLKWLICELC SLYNLPKHLD VEMLDQPLPT GQNGTTEEVT SEEEEEEEEM AEDIEDLDHY EMKEEEPISG KKSEDEGIEK ENLAILEKIR KTQRQDHLNG AVSGSVQASD RLMKELRDIY RSQSYKTGIY SVELINDSLY DWHVKLQKVD PDSPLHSDLQ ILKEKEGIEY ILLNFSFKDN FPFDPPFVRV VLPVLSGGYV LGGGALCMEL LTKQGWSSAY SIESVIMQIN ATLVKGKARV QFGANKNQYN LARAQQSYNS IVQIHEKNGW YTPPKEDG
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: UBE2Q2 ubiquitin-conjugating enzyme E2Q family member 2 [ Homo sapiens ]
• Official Symbol: UBE2Q2
• Synonyms: UBE2Q2; ubiquitin-conjugating enzyme E2Q family member 2; ubiquitin conjugating enzyme E2Q (putative) 2; ubiquitin-conjugating enzyme E2 Q2; DKFZp762C143; ubiquitin-protein ligase Q2; ubiquitin carrier protein Q2
• Gene ID: 92912
• mRNA Refseq: NM_001145335
• Protein Refseq: NP_001138807
• MIM: 612501
• UniProt ID: Q8WVN8
• Chromosome Location: 15q24.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity