Recombinant Human UBE3A Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human UBE3A Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : UBE3A-2369H
Product Overview : UBE3A MS Standard C13 and N15-labeled recombinant protein (NP_570854) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 100.1 kDa
AA Sequence : MATACKRSGE PQSDDIEASR MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM DNNAAAIKAL ELYKINAKLC DPHPSKKGAS SAYLENSKGA PNNSCSEIKM NKKGARIDFK DVTYLTEEKV YEILELCRER EDYSPLIRVI GRVFSSAEAL VQSFRKVKQH TKEELKSLQA KDEDKDEDEK EKAACSAAAM EEDSEASSSR IGDSSQGDNN LQKLGPDDVS VDIDAIRRVY TRLLSNEKIE TAFLNALVYL SPNVECDLTY HNVYSRDPNY LNLFIIVMEN RNLHSPEYLE MALPLFCKAM SKLPLAAQGK LIRLWSKYNA DQIRRMMETF QQLITYKVIS NEFNSRNLVN DDDAIVAASK CLKMVYYANV VGGEVDTNHN EEDDEEPIPE SSELTLQELL GEERRNKKGP RVDPLETELG VKTLDCRKPL IPFEEFINEP LNEVLEMDKD YTFFKVETEN KFSFMTCPFI LNAVTKNLGL YYDNRIRMYS ERRITVLYSL VQGQQLNPYL RLKVRRDHII DDALVRLEMI AMENPADLKK QLYVEFEGEQ GVDEGGVSKE FFQLVVEEIF NPDIGMFTYD ESTKLFWFNP SSFETEGQFT LIGIVLGLAI YNNCILDVHF PMVVYRKLMG KKGTFRDLGD SHPVLYQSLK DLLEYEGNVE DDMMITFQIS QTDLFGNPMM YDLKENGDKI PITNENRKEF VNLYSDYILN KSVEKQFKAF RRGFHMVTNE SPLKYLFRPE EIELLICGSR NLDFQALEET TEYDGGYTRD SVLIREFWEI VHSFTDEQKR LFLQFTTGTD RAPVGGLGKL KMIIAKNGPD TERLPTSHTC FNVLLLPEYS SKEKLKERLL KAITYAKGFG MLTRTRPLEQ KLISEEDLAA NDILDYKDDD DKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : UBE3A ubiquitin protein ligase E3A [ Homo sapiens (human) ]
Official Symbol : UBE3A
Synonyms : UBE3A; ubiquitin protein ligase E3A; EPVE6AP, HPVE6A, human papilloma virus E6 associated protein; ubiquitin-protein ligase E3A; ANCR; Angelman syndrome; AS; E6 AP; FLJ26981; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; renal carcinoma antigen NY-REN-54; human papillomavirus E6-associated protein; oncogenic protein-associated protein E6-AP; human papilloma virus E6-associated protein; E6-AP; HPVE6A; EPVE6AP;
Gene ID : 7337
mRNA Refseq : NM_130839
Protein Refseq : NP_570854
MIM : 601623
UniProt ID : Q05086

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