Recombinant Human Ubiquitin Conjugating Enzyme 9, His6-Tagged

• Cat.No.: UBE2I-03H
• Product Overview: Recombinant Human Ubiquitin Conjugating Enzyme 9 produced inE.coliis a single non-glycosylated polypeptide chain containing 171 amino acids and having a molecular mass of approximately 19.5 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Formulation: Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1×PBS, 1mM DTT, pH 7.5.
• Endotoxin: Less than 1EU/μg of rHuUbc9 as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.
• Storage: This lyophilized preparation is stable at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: UBE2I
• Synonyms: P18; UBC9; C358B7.1; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); UBC9_HUMAN; SUMO-conjugating enzyme UBC9; EC6.3.2.-; SUMO-protein ligase; Ubiquitin-protein ligase I; Ubiquitin carrier protein I; Ubiquitin carrier protein 9; UBE2I;UBCE9; Ubiquitin-protein ligase I; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Gene ID: 7329
• mRNA Refseq: NM_003345
• Protein Refseq: NP_003336
• MIM: 601661
• UniProt ID: P63279
• Chromosome Location: 16p13.3
• Function: HLH domain binding;ligase activity;protein binding; small conjugating protein ligase activity.