Recombinant Human UGT1A1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UGT1A1-1019H
• Product Overview: UGT1A1 MS Standard C13 and N15-labeled recombinant protein (NP_000454) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 59.6 kDa
• AA Sequence: MAVESQGGRPLVLGLLLCVLGPVVS HAGKILLIPVDGSHWLSMLGAIQQL QQRGHEIVVLAPDASLYIRDGAFYT LKTYPVPFQREDVKESFVSLGHNVF ENDSFLQRVIKTYKKIKKDSAMLLS GCSHLLHNKELMASLAESSFDVMLT DPFLPCSPIVAQYLSLPTVFFLHAL PCSLEFEATQCPNPFSYVPRPLSSH SDHMTFLQRVKNMLIAFSQNFLCDV VYSPYATLASEFLQREVTVQDLLSS ASVWLFRSDFVKDYPRPIMPNMVFV GGINCLHQNPLSQEFEAYINASGEH GIVVFSLGSMVSEIPEKKAMAIADA LGKIPQTVLWRYTGTRPSNLANNTI LVKWLPQNDLLGHPMTRAFITHAGS HGVYESICNGVPMVMMPLFGDQMDN AKRMETKGAGVTLNVLEMTSEDLEN ALKAVINDKSYKENIMRLSSLHKDR PVEPLDLAVFWVEFVMRHKGAPHLR PAAHDLTWYQYHSLDVIGFLLAVVL TVAFITFKCCAYGYRKCLGKKGRVK KAHKSKTHTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UGT1A1 UDP glucuronosyltransferase family 1 member A1 [ Homo sapiens (human) ]
• Official Symbol: UGT1A1
• Synonyms: UGT1A1; UDP glucuronosyltransferase 1 family, polypeptide A1; GNT1, UDP glycosyltransferase 1 family, polypeptide A1, UGT1; UDP-glucuronosyltransferase 1-1; UGT1A; UGT-1A; UGT1*1; UGT1.1; UGT1-01; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; bilirubin-specific UDPGT isozyme 1; bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; UDP glycosyltransferase 1 family, polypeptide A1; GNT1; UGT1; UDPGT; HUG-BR1; BILIQTL1; UDPGT 1-1
• Gene ID: 54658
• mRNA Refseq: NM_000463
• Protein Refseq: NP_000454
• MIM: 191740
• UniProt ID: P22309

Reviews

08/12/2022

The product was kept in perfect condition and delivered on time so that I got the perfect product in the shortest time possible.

05/14/2022

Customer service contacted me in a timely manner and helped me solve a lot of problems that arose in my experiments.

Q&As

What is the function of UGT1A1?

UGT1A1 converts lipophilic small molecules, such as drugs, into water-soluble and excretable metabolites.

What is the relationship between bilirubin and UGT1A1?

Bilirubin is a binding substrate for UGT1A1 and UGT1A1 is involved in bilirubin metabolism.

What diseases is UGT1A1 involved in?

UGT1A1 is involved in lung cancer, colorectal cancer and hyperbilirubinemia, among other diseases.

Where is UGT1A1 mainly expressed?

UGT1A1 is mainly expressed in the liver.

What are the effects of mutations in the UGT1A1 gene?

Mutations in the UGT1A1 gene prevent the glucuronidation of free bilirubin and reduces bilirubin metabolism and increases serum free bilirubin levels.