Recombinant Human UGT1A3 Protein UGT1A3-98H

Recombinant Human UGT1A3 Protein

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Recombinant Human UGT1A3 Protein

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Cat.No. : UGT1A3-98H
Product Overview : Recombinant human UGT1A3 protein without tag was expressed in insect cells.
Description : This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. Substrates of this enzyme include estrone, 2-hydroxyestrone, and metabolites of benzo alpha-pyrene.
Source : Insect cells
Species : Human
Bio-activity : UGT Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM Tris-HCl (pH 7.5) containing 5 mM magnesium chloride and 2 mM UDPGA. Alamethicin (10 μg/ml final concentration) is added to kinetics tests only)
Substrate: 17β-estradiol (3-glucuronide formation)
Linear with time up to: 20 min(Determined at 20 μM substrate, using a protein concentration of 500 μg/ml)
Linear with protein up to: 500 μg/ml(Determined at 20 μM substrate, using an incubation time of 20 min)
Vmax: 168 pmol/min/mg protein
S50: 20 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : Protein Concentration: 5 mg/ml
Storage Buffer : 25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA
Gene Name : UGT1A3 UDP glucuronosyltransferase family 1 member A3 [ Homo sapiens (human) ]
Official Symbol : UGT1A3
Synonyms : UGT1A3; UDP glucuronosyltransferase family 1 member A3; UDPGT; UGT1C; UGT-1C; UGT1.3; UGT1-03; UGT1A3S; UDPGT 1-3; UDP-glucuronosyltransferase 1A3; UDP glucuronosyltransferase 1 family, polypeptide A3; UDP glycosyltransferase 1 family, polypeptide A3; UDP-glucuronosyltransferase 1 family polypeptide A3s; UDP-glucuronosyltransferase 1-3; UDP-glucuronosyltransferase 1-C; UDP-glucuronosyltransferase 1A; UGT1*3
Gene ID : 54659
mRNA Refseq : NM_019093
Protein Refseq : NP_061966
MIM : 606428
UniProt ID : P35503
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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