| Cat.No. : |
UGT1A3-98H |
| Product Overview : |
Recombinant human UGT1A3 protein without tag was expressed in insect cells. |
| Description : |
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. Substrates of this enzyme include estrone, 2-hydroxyestrone, and metabolites of benzo alpha-pyrene. |
| Source : |
Insect cells |
| Species : |
Human |
| Bio-activity : |
UGT Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM Tris-HCl (pH 7.5) containing 5 mM magnesium chloride and 2 mM UDPGA. Alamethicin (10 μg/ml final concentration) is added to kinetics tests only)
Substrate: 17β-estradiol (3-glucuronide formation)
Linear with time up to: 20 min(Determined at 20 μM substrate, using a protein concentration of 500 μg/ml)
Linear with protein up to: 500 μg/ml(Determined at 20 μM substrate, using an incubation time of 20 min)
Vmax: 168 pmol/min/mg protein
S50: 20 μM |
| Usage : |
For laboratory (research) purposes only |
| Storage : |
Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
| Concentration : |
Protein Concentration: 5 mg/ml |
| Storage Buffer : |
25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.