| Description : |
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
53.3 kDa |
| AA Sequence : |
MAGPGWGPPRLDGFILTERLGSGTY ATVYKAYAKKDTREVVAIKCVAKKS LNKASVENLLTEIEILKGIRHPHIV QLKDFQWDSDNIYLIMEFCAGGDLS RFIHTRRILPEKVARVFMQQLASAL QFLHERNISHLDLKPQNILLSSLEK PHLKLADFGFAQHMSPWDEKHVLRG SPLYMAPEMVCQRQYDARVDLWSMG VILYEALFGQPPFASRSFSELEEKI RSNRVIELPLRPLLSRDCRDLLQRL LERDPSRRISFQDFFAHPWVDLEHM PSGESLGRATALVVQAVKKDQEGDS AAALSLYCKALDFFVPALHYEVDAQ RKEAIKAKVGQYVSRAEELKAIVSS SNQALLRQGTSARDLLREMARDKPR LLAALEVASAAMAKEEAAGGEQDAL DLYQHSLGELLLLLAAEPPGRRREL LHTEVQNLMARAEYLKEQVKMRESR WEADTLDKEGLSESVRSSCTLQTRT RPLEQKLISEEDLAANDILDYKDDD DKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
