Recombinant Human URM1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: URM1-298H
• Product Overview: URM1 MS Standard C13 and N15-labeled recombinant protein (NP_112176) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Acts as a sulfur carrier required for 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm5S2U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 11.4 kDa
• AA Sequence: MAAPLSVEVEFGGGAELLFDGIKKH RVTLPGQEEPWDIRNLLIWIKKNLL KERPELFIQGDSVRPGILVLINDAD WELLGELDYQLQDQDSVLFISTLHG GTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: URM1 ubiquitin related modifier 1 [ Homo sapiens (human) ]
• Official Symbol: URM1
• Synonyms: URM1; ubiquitin related modifier 1; C9orf74, chromosome 9 open reading frame 74, ubiquitin related modifier 1 homolog (S. cerevisiae); ubiquitin-related modifier 1 homolog; MGC2668; ubiquitin related modifier 1 homolog; C9orf74; RP11-339B21.4
• Gene ID: 81605
• mRNA Refseq: NM_030914
• Protein Refseq: NP_112176
• MIM: 612693
• UniProt ID: Q9BTM9