Recombinant Human Uroplakin 3A, His-tagged

• Cat.No.: UPK3A-4747H
• Product Overview: Recombinant human UPK3A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: UPK3A belongs to the uroplakin-3 family. Uroplakins (UP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (UPII, UPIIIa, and UPIIIb) or four times (UPIa and UPIb; both belonging to the "tetraspanin" family). All have large luminal/extracellular domains, but only UPIIIa and UPIIIb have significant cytoplasmic portions in their C-termini. UPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl
• Molecular Weight: 23.1kDa (214aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UPK3A uroplakin 3A [ Homo sapiens ]
• Official Symbol: UPK3A
• Synonyms: UPK3A; UP3A; UPK3; UPIII; UPIIIA; MGC119178; uroplakin-3a; uroplakin III; OTTHUMP00000199859; OTTHUMP00000199860; uroplakin 3A; Uroplakin III; uroplakin 3; UP3a
• Gene ID: 7380
• mRNA Refseq: NM_006953
• Protein Refseq: NP_008884
• MIM: 611559
• UniProt ID: O75631
• Chromosome Location: 22q13.31