| Cat.No. : |
USP14-01H |
| Product Overview : |
Recombinant Human USP14 Protein without tag was expressed in E. coli |
| Description : |
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Source : |
E. coli |
| Species : |
Human |
| Bio-activity : |
Recombinant Human USP14 is a Ubiquitin-specific deconjugating enzyme. The activity of Recombinant Human USP14 was measured at 2.0 RFU/min/μg using an enzyme concentration of 18 μM and a Ub-AMC concentration of 4 μM in 100 mM HEPES, pH 8.5, 10 mM DTT, and 5% Glycerol at 25 degreesC. USP14 activity is greatly stimulated in the presence of 19S proteasome. |
| Molecular Mass : |
56 kDa |
| Purity : |
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 degreesC as supplied. 3 months, -70 degreesC under sterile conditions after opening. |
| Storage Buffer : |
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 50 mM NaCl, 5 mM DTT, 1 mM EDTA |
| Shipping : |
The product is shipped with dry ice or equivalent. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.