- Human(7)
- Rhesus Macaque(1)
- Zebrafish(1)
Recombinant Human USP14 Protein
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| Cat.No. : | USP14-01H |
| Product Overview : | Recombinant Human USP14 Protein without tag was expressed in E. coli |
| Description : | This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Source : | E. coli |
| Species : | Human |
| Bio-activity : | Recombinant Human USP14 is a Ubiquitin-specific deconjugating enzyme. The activity of Recombinant Human USP14 was measured at 2.0 RFU/min/μg using an enzyme concentration of 18 μM and a Ub-AMC concentration of 4 μM in 100 mM HEPES, pH 8.5, 10 mM DTT, and 5% Glycerol at 25 degreesC. USP14 activity is greatly stimulated in the presence of 19S proteasome. |
| Molecular Mass : | 56 kDa |
| Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
| Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 degreesC as supplied. 3 months, -70 degreesC under sterile conditions after opening. |
| Storage Buffer : | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 50 mM NaCl, 5 mM DTT, 1 mM EDTA |
| Shipping : | The product is shipped with dry ice or equivalent. |
| Gene Name : | USP14 ubiquitin specific peptidase 14 [ Homo sapiens (human) ] |
| Official Symbol : | USP14 |
| Synonyms : | USP14; ubiquitin specific peptidase 14; TGT; ubiquitin carboxyl-terminal hydrolase 14; deubiquitinating enzyme 14; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14 (tRNA-guanine transglycosylase); ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; EC 3.4.19.12 |
| Gene ID : | 9097 |
| mRNA Refseq : | NM_005151 |
| Protein Refseq : | NP_005142 |
| MIM : | 607274 |
| UniProt ID : | P54578 |
◆ Recombinant Protein
| USP14-3617H | Recombinant Human USP14, GST-tagged | +Inquiry |
| USP14-159H | Recombinant Human USP14, His-tagged | +Inquiry |
| USP14-10337Z | Recombinant Zebrafish USP14 | +Inquiry |
| USP14-7221H | Recombinant Human USP14, His-tagged | +Inquiry |
| USP14-147H | Recombinant Human USP14, His-SUMO-tagged | +Inquiry |
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◆ Lysates
| USP14-471HCL | Recombinant Human USP14 293 Cell Lysate | +Inquiry |
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USP14USP15USP2USP7USP17L2USP3USP31USP33USP34USP39USP4USP47USP48USP5USP9YUSP13USP38USP10USP20USP21USP26USP6USP9XUSP29USP43USP45USP22USP19USP18USP50USP53USP6NLUSP30USP32USP11USP12USP46USP24USP35USP1USP25USP28USP36USP37USP49USP8USPUSP40USP44Usp16Usp17l5Usp27xUsp42Usp51Usp54
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