Recombinant Human USP2 Protein, His-tagged USP2-011H

Recombinant Human USP2 Protein, His-tagged

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Recombinant Human USP2 Protein, His-tagged

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Cat.No. : USP2-011H
Product Overview : Recombinant Human HSPA4 protein (259-605, active catalytic domain) with a C-terminally Histidine tag was expressed in E. coli.
Description : This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Source : E. coli
Species : Human
Tag : His
Form : 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 1 mM DTT, and 0.1 mM EDTA
Molecular Mass : 40.9 kDa
Protein length : 259-605
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of USP2 enzyme required to produce 1 pmol of AMC per minute at room temperature in 50 mM HEPES, pH 7.8, 0.5 mM EDTA, 1 mM DTT,and 0.2% CHAPS
Stability : ≥ 1 year
Storage : Store at -80 centigrade
Shipping : Dry ice in continental US; may vary elsewhere
SDS-PAGE analysis of USP2. :

Lane 1: MW Markers
Lane 2: USP2 (1 µg)
Lane 3: USP2 (2 µg)
Lane 4: USP2 (4 µg)

ATPase Activity of USP2 :

Gene Name : USP2 ubiquitin specific peptidase 2 [ Homo sapiens (human) ]
Official Symbol : USP2
Synonyms : USP2; ubiquitin specific peptidase 2; USP9; UBP41; ubiquitin carboxyl-terminal hydrolase 2; 41 kDa ubiquitin-specific protease; deubiquitinating enzyme 2; ubiquitin specific protease 12; ubiquitin specific protease 9; ubiquitin thioesterase 2; ubiquitin-specific-processing protease 2; EC 3.4.19.12
Gene ID : 9099
mRNA Refseq : NM_001243759
Protein Refseq : NP_004196
MIM : 604725
UniProt ID : O75604

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