Recombinant Human USP9X Protein (K1554-N1995), GST tagged

• Cat.No.: USP9X-0343H
• Product Overview: Recombinant Human GST-TEV-GG-USP9X(K1554-N1995) Protein was expressed in E. coli.

Specification

• Description: Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-63'-, 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Deubiquitinates mTORC2 complex component RICTOR at 'Lys-294' by removing 'Lys-63'-linked polyubiquitin chains, stabilizing RICTOR and enhancing its binding to MTOR, thus promoting mTORC2 complex assembly. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration. Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein ARNTL/BMAL1 via its deubiquitinating activity. Deubiquitinates PEG10.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Liquid
• Protein Length: K1554-N1995
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: USP9X ubiquitin specific peptidase 9, X-linked [ Homo sapiens (human) ]
• Official Symbol: USP9X
• Synonyms: USP9X; ubiquitin specific peptidase 9, X-linked; ubiquitin specific peptidase 9, X linked (fat facets like, Drosophila), ubiquitin specific protease 9, X chromosome (fat facets like Drosophila), ubiquitin specific protease 9, X linked (fat facets like, Drosophila); probable ubiquitin carboxyl-terminal hydrolase FAF-X; DFFRX; FAF; hFAM; fat facets in mammals; ubiquitin thioesterase FAF-X; deubiquitinating enzyme FAF-X; ubiquitin thiolesterase FAF-X; fat facets protein related, X-linked; fat facets protein-related, X-linked; Drosophila fat facets related, X-linked; ubiquitin-specific protease 9, X chromosome; ubiquitin-specific processing protease FAF-X; ubiquitin-specific-processing protease FAF-X; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); FAM
• Gene ID: 8239
• mRNA Refseq: NM_001039590
• Protein Refseq: NP_001034679
• MIM: 300072
• UniProt ID: Q93008