Recombinant Human VHL protein, His-Avi-tagged

• Cat.No.: VHL-2220H
• Product Overview: Recombinant Human VHL protein(NP_000542.1)(Met1-Asp213) is expressed in E.coli, fused with a 6xHis tag at the N-terminus and an AVI tag at the C-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: Avi&His
• Protein Length: 1-213 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, pH 7.5.
• Molecular Mass: 27.5 kDa
• Purity: ≥ 75 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8℃ for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: VHL von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: VHL
• Synonyms: VHL; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase; von Hippel Lindau syndrome , von Hippel Lindau tumor suppressor; von Hippel-Lindau disease tumor suppressor; VHL1; protein G7; elongin binding protein; RCA1; pVHL; HRCA1
• Gene ID: 7428
• mRNA Refseq: NM_000551
• Protein Refseq: NP_000542
• MIM: 608537
• UniProt ID: P40337